Agrobacterium-mediated Transformation of Squash (Cucurbita pepo L.) to induce Virus Resistance by Gene Silencing
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چکیده
منابع مشابه
Agrobacterium-mediated transformation of cotyledonary leaf of lettuce (Lactuca sativa L.) by the GCHI gene
Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...
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Squash leaf curl virus (SqLCV) is a bipartite begomovirus affecting squash plants. It is transmitted by whitefly Bemisia tabaci biotype B causing severe leaf curling, vein banding, and molting ending by stunting. In this study full-length genomic clone of SqLCV Egyptian isolated and posttranscriptional gene silencing (PTGS) has been induced to develop virus resistance. The Noubaria SqLCV has mo...
متن کاملl-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.
ORNITHINE: 2-oxoacid aminotransferase (EC 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (Cucurbita pepo, L.) seedlings. The pH optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the Michaelis constants are 4.7 mm and 6.3 mm, respectively. The enzyme has a molecular weight of 48,000 ...
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René Renato Balandrán-Quintana1, Ana María Mendoza-Wilson2, Irasema Vargas-Arispuro2 and Miguel Angel Martínez-Téllez2* Universidad Autónoma de Chihuahua, Facultad de Ciencias Químicas, Ciudad Universitaria s/n, A.P. 1542-C Chihuahua, Chihuahua, México Centro de Investigación en Alimentación y Desarrollo, A.C., Coordinación de Tecnología de Alimentos de Origen Vegetal, Km 0.6, Carretera a La Vi...
متن کاملAgrobacterium Mediated Transformation of Maize (Zea mays L.)
Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
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ژورنال
عنوان ژورنال: Journal für Verbraucherschutz und Lebensmittelsicherheit
سال: 2006
ISSN: 1661-5751,1661-5867
DOI: 10.1007/s00003-006-0086-1